Table 1.

Gene Locus, Protein name, NCBI Reference Sequence, Features, Gene Conservation, Sequence of the Primers Employed for DNA Amplification, and Molecular Mass of Expressed Recombinant Proteins

Gene Locus1	Recombinant Protein given Name	NCBI Reference Sequence Number2	Description/Function	Conservation (Identity)3	Sequence of primers for PCR amplification	Recombinant Protein Molecular Mass
LIC10368	Lsa21b	YP_000355	Putative lipoprotein	Lai (100 %)	F: 5´ CACCGATGAAAAAAAAGAAAATGAATTGAG 3’ R: 5’ AACGCGATTCATAGAGAGCG 3’	21 kDa
LIC10258	Lsa66a	YP_000249	Hypothetical protein with ompA domain	Lai (99 %) LBH (83 %)	F:5´ GGATCCGAAGCCTTCTCACCCAATTG 3´ (BamH I) R:5´ CCATGGTTAAAGTGAAAGATAAAAATCGATTC 3´ (Nco I)	65 kDa
LIC11030	rLIC11030c	YP_001000	Putative lipoprotein with a domain of unknown function (DUF1565)	Lai (99 %)	F: 5` CTCGAGCCAATTTCTTTCGATCCAAATC 3´ (XhoI) R: 5`AAGCTTTCAATCCTCTACTGCAGCCC 3´ (HindIII)	37 kDa
LIC10821	rLIC10821	YP_000798	Putative lipoprotein with a domain of unknown function (DUF1565)	Lai (100 %)	F: 5´ CTCGAGTGTGCCAACGAAAACG 3´ (XhoI) R: 5´ AAGCTTTCAAGGATTACAAGGTTTAG 3´ (HindIII)	37 kDa
LIC12253	Lsa25d	YP_002188	Putative lipoprotein with a domain of unknown function (DUF1566)	Lai (100 %) LBH (77 %) LBP (39 %)	F: 5´ CTCGAGGAGGAGAAACCGGACGATAC 3’ (XhoI) R: 5’ CCATGGTTAGGGAAGACTTCTAACACATC 3’ (NcoI)	24 kDa
LIC10672	rLIC10672	YP_000656	hypothetical protein	Lai (100 %) LBH (95 %)	F: 5´CCGGATCCCAGAATAAAG 3´(BamHI) R: 5´GGGGTACCTCAGATGTCG 3´(KpnI)	23 kDa

¹http://aeg.lbi.ic.unicamp.br/world/lic/ [30, 41].

²http://www.ncbi.nlm.nih.gov/protein/.

³Protein BLAST - http://www.ncbi.nlm.nih.gov/blast/Blast.cgi [42, 43].

^aPreviously published by Oliveira et al [26].

^bPreviously published by Atzingen et al [24]

^cPreviously published by Mendes et al [25]

^dPreviously published by Felix et al. [21]; Domingos et al., [27].

Lai: L. interrogans serovar Lai [44]; LBH: L. borgpetersenii serovar Hardjo-bovis [45]; LBP: L. biflexa serovar Patoc [46].