Fig 3.

Expression of xylB_ADP1 in A. baylyi ADP1 cells assessed by RT-PCR analysis using 37.5 ng RNA from cells incubated in MSM medium as the template in the presence of 5 mM benzyl alcohol for 3 h (lane 1), 1 mM coniferyl alcohol for 3 h (lane 2), 1% (wt/vol) sodium gluconate under nitrogen-saturated conditions (0.1% [wt/vol] NH₄Cl) for 5 h (lane 3), and 1% (wt/vol) sodium gluconate under nitrogen-limited conditions (0.01% [wt/vol] NH₄Cl) for 5 h (lane 4). Lane 5, 1-kb DNA ladder; bands correspond to 120 ng DNA (1,000 bp) or 50 ng DNA (750, 500, and 250 bp).