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Purification of recombinant aryl-alcohol dehydrogenase XylBADP1 found in A. baylyi ADP1 from E. coli BL21(DE3) pET23::xylBADP1
| Purification step | Total activity (U) | Total protein (mg) | Sp act (U min−1) | Purification (fold) | Yield (%) |
|---|---|---|---|---|---|
| Crude extract | 8,520 | 1,975 | 4.1 | 1.0 | 100.0 |
| Soluble fraction | 8,674 | 1,883 | 4.7 | 1.1 | 101.8 |
| Q-Sepharose | 4,630 | 386 | 12.0 | 2.9 | 54.3 |
| Octyl Sepharose | 2,822 | 121 | 23.3 | 5.6 | 33.1 |
| Ultrafiltration | 2,576 | 111 | 23.2 | 5.6 | 30.2 |
| Cibacron F3GA Sepharose CL6-B | 3,010 | 55.4 | 54.4 | 13.2 | 35.3 |
