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SDS-PAGE analysis of purified wild-type and mutant meso-DAPDHs from S. thermophilum. (A) Purification of wild-type meso-DAPDH. Lane M, protein ruler; lane 1, crude enzyme extract; lane 2, enzyme purified by affinity chromatography. (B) Purified wild-type and mutant meso-DAPDHs by affinity chromatography. Lane M, protein ruler (35.0 kDa); lane 1, wild type; lane 2, F146W; lane 3, M152Q; lane 4, F146W M152Q.
