Table 1.
B. subtilis strains used in this study
| Strain | Genotype and/or phenotypea | Missing spore component(s) | Source (reference) |
|---|---|---|---|
| 168 (WN131) | Wild-type parent of WN659 and WN661; trpC2 | None | Laboratory stock (4) |
| PS832 (WN552) | Wild-type parent of PS356, PS1899, FB72, and FB108; prototroph | None | P. Setlow (44) |
| PY79 (WN470) | Wild-type parent of AD17, AD28, and AD142; prototroph | None | A. Driks (34) |
| AD17 (WN496) | gerE36 | Inner coat | A. Driks (34) |
| AD28 (WN495) | cotE::cat Cmr | Outer coat | A. Driks (34) |
| AD142 (WN469) | gerE36 cotE::cat Cmr | Inner and outer coats | A. Driks (34) |
| PS356 (WN1273) | ΔsspA ΔsspB | SASP-α and SASP-β | P. Setlow (17) |
| PS1899 (WN1274) | dacB::cat Cmr | Core dehydration | P. Setlow (33) |
| FB72 (WN552) | ΔgerA::spc ΔgerB::cat ΔgerK::erm Spcr Cmr Ermr (referred to as ΔgerABK DPA+ spores) | Germination receptors | P. Setlow (44) |
| FB108 (WN553) | ΔgerA::spc ΔgerB::cat ΔgerK::erm ΔspoVF::tet Spcr Cmr Ermr Tetr (referred to as ΔgerABK DPA− spores) | Germination receptors, DPA | P. Setlow (44) |
| WN661 | trpC2, presumed mutation in cotAb | Pigmentation | W. L. Nicholson (unpublished data) |
a
Cmr, resistant to chloramphenicol (5 μg/ml); Ermr, resistant to erythromycin (1 μg/ml); Spcr, resistant to spectinomycin (100 μg/ml); Tetr, resistant to tetracycline (10 μg/ml).
b
Reference 13.