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Fluorescence in situ hybridization of the enrichment culture at different times of incubation. (A) Month 9; (B) month 14; (C) month 19. NC10 bacteria appear pink due to cohybridization of NC10 bacterium-specific probes 193-Cy3 and 1027-Cy3 (red) and a mixture of probes EUBI to III and IV-Cy5 (light blue) for most eubacteria and DAPI (dark blue). (Scale bars: 5 μm).
