Table 1.
Primer pairs used for qPCR analysis and clone library construction
| Designationa and purpose | Sequence (5′–3′) | Reference | Annealing temp (°C) | Target |
|---|---|---|---|---|
| qPCR analysis | ||||
| p1F | GGGCTTGACATCCCACGAACCTG | 14 | 65 | NC10 bacterial 16S rRNA |
| p1R | CGCCTTCCTCCAGCTTGACGC | 14 | ||
| p2F | GGGGAACTGCCAGCGTCAAG | 14 | 65 | NC10 bacterial 16S rRNA |
| p2R | CTCAGCGACTTCGAGTACAG | 14 | ||
| 533F | GTGCCAGCMGCCGCGGTAA | 49 | 58 | All bacterial 16S rRNA |
| 805R | GACTACCAGGGTATCTAATC | 28 | ||
| 1100F | YAACGAGCGCAACCC | 10 | 58 | All bacterial 16S rRNA |
| 1492R | GGTTACCTTGTTACGACTT | 53 | ||
| Clone library construction | ||||
| 8F | AGAGTTTGATYMTGGCTCAG | 21 | 55–65 | NC10 bacterial 16S rRNA |
| 193F | GACCAAAGGGGGCGAGCG | 14 | ||
| 1043R | TCTCCACGCTCCCTTGCG | 14 | ||
| A189bF | GGNGACTGGGACTTYTGG | 34 | 55–65 | NC10 bacterial pmoA |
| cmo182F | TCACGTTGACGCCGATCC | 34 | ||
| cmo682R | AAAYCCGGCRAAGAACGA | 34 |
a
F, forward; R, reverse.