TABLE 1.
PCR amplification primers used for full-length Ion Torrent analysis of M. tuberculosis genes
| Amplification target | Forward primer | Reverse primer | Amplicon length (bp) |
|---|---|---|---|
| rpoB | 5′-TCCTCTAAGGGCTCTCGTT-3′ (19 nta) | 5′-GTCAGGTACACGATCTCGT-3′ (19 nt) | 1,625 |
| rpoBII (second half) | 5′-ATCGAAACGCCGTACCGCAA-3′ (20 nt) | 5′-TGACGTCGAGCACGTAACTCCCT-3′ (23 nt) | 2,056 |
| katG | 5′-ACACCAACTCCTGGGAAGGAAT-3′ (21 nt) | 5′-TGATCGCACATCCAGCACATTT-3′ (22 nt) | 2,447 |
| pncA | 5′-GACGGATTTGTCGCTCACTAC-3′ (21 nt) | 5′-GCCGGAGACGATATCCAGAT-3′ (20 nt) | 960 |
| gyrA | 5′-AAGGATGTTCGGTTCCTGGAT-3′ (21 nt) | 5′-TAACACTCGTACCCGGCT-3′ (18 nt) | 2,664 |
| rrs (16S) | 5′-TTCTAAATACCTTTGGCTCCCT-3′ (22 nt) | 5′-TGGCCAACTTTGTTGTCATGCA-3′ (22 nt) | 1,680 |
| Total | 11,432 total (5 genes) |
a
nt, nucleotides.