TABLE 2.
No. of isolates | Amino acid substitution(s)b yielded by the rpoB gene (3,619 bp) | Rifampin result by: |
||
---|---|---|---|---|
Ion Torrentc | Hain LPA | Bactec MGIT 960 | ||
9 | S531L | Resistant | Resistant | Resistant |
1 | S531L, V194I | Resistant | Resistant | Resistant |
1 | S531L, Y645H | Resistant | Resistant | Resistant |
1 | H526D | Resistant | Resistant | Resistant |
1 | H526Y, S509R | Resistant | Resistant | Resistant |
1 | H526R | Resistant | Resistantd | Resistant |
5 | Wild typeb | Sensitive | Sensitive | Sensitive |
6 | D516G, L533P | Resistant | Resistantd | Resistant |
1 | D516V | Resistant | Resistant | Resistant |
There were 5 rpoB mutations yielding amino acid substitutions (R908C, Q1042H, P1043A, I1187T, and V1249F) noted in at least 1 strain at the 3′ end (residues 900 to 1253).
Compared to the H37Rv reference strain.
Rifampin resistance is known to occur in rpoB at positions corresponding to amino acids 531(S→T), 526 (H→Y/D), and 516 (D→V).
Using Hain LPA, strains with an uncommon glycine substitution at position 516 and an arginine at position 526 were indirectly determined as resistant by absence of a wild-type band.