TABLE 6.
Summary of 4 amino acid mutations in the rrs (16S) gene products of 26 (14 MDR, 7 XDR, and 5 fully susceptible) M. tuberculosis isolates from South Africa deduced by Ion Torrent sequencing and culture
| No. of isolates | Amino acid substitution(s)a in the rrs (16S) gene (1,680 bp) | Kanamycin result by: |
|
|---|---|---|---|
| Ion Torrentb | Bactec MGIT 960 | ||
| 1 | G878A | Sensitive | Sensitive |
| 12 | Wild typea | Sensitive | Sensitive |
| 1 | A514C, A1401G | Resistant | Resistant |
| 6 | A1401G | Resistant | Resistant |
| 3 | A514C | Sensitive | Sensitive |
| 1 | C492T | Sensitive | Sensitive |
| 1 | C492T, A514C | Sensitive | Sensitive |
| 1 | A514C | Sensitive | Sensitive |
a
Compared to the H37Rv reference strain.
b
Aminoglycoside resistance is known to occur at positions 1401 (A→G), 1402 (C→T), and 1484 (G→T) (indicated in bold).