TABLE 4.

Results from the SETS swab extraction protocol by culture and real-time PCR using lesion swabs from cutaneous anthrax outbreaks^a

Specimen IDb	Culture result and growth characteristic	Average CT (mean ± SD)c
		Chromosome	pXO1	pXO2
BCA00001	−, no growth	35.9 ± 0.52	34.9 ± 0.72	35.6 ± 0.63
BCA00004	−, no growth	38.2 ± 0.49	37.8 ± 0.71	36.9 ± 1.37
BCA00007	−, non-B. anthracis	37.3 ± 0.67	39.0 ± 0.55	39.0 ± 0.55
BCA00009	−, non-B. anthracis	25.7 ± 0.17	24.4 ± 0.22	24.5 ± 0.02
BCA00010	−, non-B. anthracis	37.4 ± 1.14	35.0 ± 0.45	29.5 ± 0.67
BCA00011	−, non-B. anthracis	31.6 ± 0.37	34.6 ± 0.90	36.6 ± 0.48
BCA00012	−, non-B. anthracis	34.9 ± 0.90	31.7 ± 0.77	31.5 ± 0.35
BCA00014	−, non-B. anthracis	36.1 ± 1.01	33.8 ± 0.03	34.5 ± 0.49
BCA00015	−, non-B. anthracis	31.7 ± 0.48	29.4 ± 0.29	30.1 ± 0.04
BCA00016	−, non-B. anthracis	31.3 ± 0.23	29.2 ± 0.14	29.8 ± 0.35
BCA00017	−, non-B. anthracis	32.4 ± 0.25	30.7 ± 0.34	31.2 ± 0.14
BCA00018	−, non-B. anthracis	38.1 ± 0.09	37.2 ± 0.94	38.3 ± 0.55
BCA00019	−, no growth	34.6 ± 0.34	33.0 ± 0.15	34.0 ± 0.20
BCA00022	−, no growth	28.2 ± 0.14	26.5 ± 0.04	26.7 ± 0.14
BCA00023	−, non-B. anthracis	30.6 ± 0.05	28.1 ± 0.10	29.0 ± 0.09

^aOutbreaks in Bangladesh in 2009.

^bIdentification (ID) numbers were assigned to deidentify patient specimens. The specimens were processed using the SETS method and were tested for isolation of B. anthracis in culture as well as automated DNA purification with the MagNA Pure Compact, followed by real-time PCR analysis.

^cAverage C_T values (mean ± standard deviation) are shown only for triplicate DNA samples from specimens that were positive for all three targets in the B. anthracis real-time PCR assay described by Hoffmaster et al. (16). PCR-negative results from eight swabs are not shown.