Figure 4.

Western analysis showing the abundance of phytoene synthase and phytoene desaturase during high-light treatment. Equal amounts of protein (30 μg per lane) were separated and electro-transferred as described in the experimental procedures. Primary antibodies were anti-phytoene synthase antibodies (A) and anti-phytoene desaturase antibodies (B). Secondary antibodies were linked to horseradish peroxidase. Detection was performed using the enhanced chemiluminescence system. C, The biotin-containing bands showing an increase during high-light treatment represent an internal control and were detected using alkaline phosphatase-conjugated streptavidin. Ct, Control (N. pseudonarcissus chromoplast extract).