TABLE 1.
Chromosomal positions of the six polymorphic loci identified in L. (V.) braziliensis from Corte de Pedraa
| Locus (band)b | Primers used for locus amplification by PCR | Chromosome | Location in chromosome | Melting (annealing) temp (°C) | [MgCl2] (mM) | Amplicon size (bp) | Gene product overlapped |
|---|---|---|---|---|---|---|---|
| CHR 24/3074 (B1) | 5′-GGACTGGAGTGATCGAA, 5′-TGGCTCAAGTGTCGCA | 24 | 3074–3848 | 55 | 1.0 | 779 | Pyruvate dehydrogenase kinase |
| CHR 26/765c (B2) | 5′-GAAATAAGAGACGAAAAGGC, 5′-TGACGTAGGGGTAGAGAAAC | 26 | 765–1425 | 58 | 1.0 | 660 | |
| CHR 28/195696 (B3) | 5′-GGACTGGAGTCTGCTTTATTTG, 5′-ACCAGGGTCGTGAACATGCT | 28 | 195696–196300 | 55 | 1.5 | 618 | Nucleobase/nucleoside transporter |
| CHR 28/425451 (B4) | 5′-TAAGGTGAACAAGAAGAATC, 5′-CTGCTCGCTTGCTTTC | 28 | 425451–426067 | 48 | 1.0 | 622 | Long-chain fatty acid-coenzyme A ligase protein |
| CHR 32/1356278 (B5) | 5′-GTACGCACATGAGCCCGGAT, 5′-GCGGCGTATCACGCACTTTT | 32 | 1356278–1356777 | 55 | 2.5 | 498 | |
| CHR 35/335652 (B6) | 5′-TTATAGACGTGACACAGCG, 5′-AGCAGTCCGGAGTTGT | 35 | 335652–336148 | 56 | 1.0 | 497 |
a
The complete L. (V.) braziliensis MHOM/BR/75/M2904 genome, accessed at the http://blast.ncbi.nlm.nih.gov/Blast.cgi website, was used.
b
Corresponding band in Fig. 1.
c
This locus is duplicated in chromosome 33 at position 1476284.