Fig 4.

Plaque phenotypes of mutant viruses at p0, p5, and p10 in BHK-21 cells. BHK-21 cells were infected with wtBUNV or different minimal genome recombinant BUNVs. Cells were fixed at 3 days postinfection in 4% formaldehyde and immunostained for N protein (p0) or stained with Giemsa's solution (p5 and p10).