Fig 4.

Analysis of antigen-specific production of cytokines by CD8⁺ T cell memory populations. PBMCs isolated directly ex vivo were stimulated overnight with Gag or Nef peptides. The percentage of IFN-γ (left), TNF-α (center), and IFN-γ and TNF-α dually positive CD8⁺ T cells (right) for each memory population was calculated. Results are shown as the percentage of CD8⁺ T cells for each memory subpopulation for each patient. (A) Gag stimulation of ES cells (n = 7) is shown at the top, and Nef stimulation is shown at the bottom. (B) Gag stimulation of CP cells (n = 8) is shown at the top, and Nef stimulation is shown at the bottom. The median value of the normalized percent inhibition for each subpopulation is indicated. Only significant P values are indicated.