Fig 1.

PB2 and NP outcompete Mx1 activity. HEK293T cells were transfected in triplicate with PB1, PB2, PA, and NP expression plasmids (25 ng each), together with pHW-NSLuc (100 ng) and pRL-CMV (25 ng). In addition, 125 ng of pCAXL (A) or pCAXL-Mx1 (B) was cotransfected. Increasing amounts of PB1, PB2, PA, or NP were introduced by increasing the amount of the corresponding expression vectors (125 ng, 250 ng, or 625 ng). The normalized luciferase activity in the lysates was determined 48 h after transfection. Bars represent the average of the triplicates, and the error bars depict one standard deviation. This graph is representative of three independent experiments. Mx1, NP, and actin expression were determined in the lysates by Western blotting.