Fig 6.
Blockage of viral protein expression by LY294002 is caspase dependent. (A) The percentage of cell viability after treatment with 40 μM zVAD.fmk alone or together with 20 μM LY294002 was measured by the XTT assay. The cell viability in untreated controls at 48 h was defined as 100% survival. Values are means ± SD from three independent experiments. (B) PK15 cells were preincubated for 1 h with the pancaspase inhibitor zVAD.fmk (40 μM) in either the absence or the presence of LY294002 (LY) (20 μM). Cells were then infected with PCV2 at an MOI of 1 TCID50, and cell lysates were collected and subjected to Western blotting with using anti-ORF2 antibody. The pancaspase inhibitor zVAD.fmk reverses the blockage of LY294002 upon viral protein expression. Anti-β-actin antibody was used as an internal control for protein loading.