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. 2012 Dec;86(24):13253–13262. doi: 10.1128/JVI.01432-12

Fig 5.

Fig 5

Dominant-negative JNK expression blocks MHV68 gene expression. HEK293T cells were transfected with infectious MHV68 BAC DNA in combination with empty vector, DN-JNK1, DN-JNK2, or both DN-JNK1 and DN-JNK2. Cells were harvested 48, 72, and 96 h posttransfection, and lysates were resolved by SDS-PAGE. Resolved proteins were detected by immunoblot analyses using antibodies that recognize the indicated proteins. Note that DN-JNK constructs encode a FLAG epitope tag. DN-JNK1 and DN-JNK2 proteins were detected using FLAG-specific antibodies.