Fig 6.

Pathogenesis of rMA15 in LysM/STAT1 and FoxJ1/STAT1 mice. (A) PCR confirmation for LysM/STAT1 mice showing STAT1 deletion only in monocytes and macrophages and not in blood of transgenic mice used for the experiments. The arrow indicates the WT STAT1 band, and the arrowhead indicates a deleted STAT1 band. (B) Immunohistochemical labeling of STAT1 in WT, STAT1^−/−, and FoxJ1/STAT1 mice to confirm deletion of STAT1 in ciliated epithelial cells. Lung sections were stained with anti-STAT1 antibody. The bright red color is positive staining with antibody. Note in the FoxJ1/STAT1 image that alveolar cells and Clara cells are positive but ciliated epithelial cells are negative for staining. (C) Weight loss curve of mice infected with rMA15 (n = 5 for each time point). The error bars indicate standard deviations. (D) Histological scoring of H&E-stained lung sections in panel E. (E) H&E staining of WT, STAT1^−/−, and LysM/STAT1 mouse lungs after infection with rMA15 or mock infection with PBS control at 9 days p.i.