Fig 2.

Anti-Ag85A-specific IgM humoral immune response. Nine- to ten-week-old BALB/c mice had been vaccinated by intramuscular injection (caudal thigh muscle) of 1 × 10¹⁰ genomic particles of the indicated vectors (n = 6). Of note, all preparations were adjusted for equal numbers of capsids (Fig. 1). An additional group of mice (n = 6), which had been injected with 10 μg of recombinant Ag85A protein subcutaneously, served as a positive control. Sera of BALB/c mice collected prior to injection (not shown) and 2 (A), 4 (B) and 8 (C) weeks p.i. were assayed for anti-Ag85A antibodies by ELISA. On the y axes, anti-Ag85A IgM levels are displayed as absorbance at 450 nm (sera diluted 1:50). Error bars indicate the standard error of the mean (unpaired two-tailed t test). ***, P ≤ 0.001; **, P = 0.01; *, P < 0.05; NS, not significant.