Fig 6.

Booster vaccination with rAg85A. Anti-Ag85A IgG titers in sera of mice vaccinated with the vectors described above (Fig. 1) or rAg85A were measured 13 weeks p.i. by ELISA. Next, all mice received a subcutaneous booster vaccination with 10 μg of rAg85A. Two weeks p.i., sera were assayed for anti-Ag85A IgG titers by ELISA. On the y axes, anti-Ag85A IgG antibody titers are displayed as absorbance at 450 nm. Error bars indicate the standard error of the mean. Booster IgG responses were significantly higher for both capsid-modified vectors in comparison with AAV:Ag85A (Ag85A-AAV:Ag85A versus AAV:Ag85A, P = 0.0416, and Ag85A-AAV:GFP versus AAV:Ag85A, P < 0.005, unpaired two-tailed t test; dilution factor, 1:5,000).