Fig 6.

Effects of genistein and okadaic acid on JEV entry. (A and C) B104 cells were pretreated with genistein (A) or okadaic acid (C) and infected with JEV. After 1 h of internalization, extracellular virus was inactivated with proteinase K and the cell pellets were plated onto B104 cells to determine internalized virus by an infectious center assay. Bar graphs represent the percentage of internalized virus with respect to that of a control without drug treatment. Cell viability upon drug treatments was unaffected, as represented by the line graphs. Results are presented as the means ± SD of three independent experiments. (B and D) B104 cells preincubated with genistein (B) or okadaic acid (D) were inoculated with JEVpv or VSVpv. GFP-positive cells were determined by flow cytometry. Results are expressed as infectious units (IU)/milliliter and are presented as the means ± SD of three independent experiments. *, P < 0.05.