Fig 7.

Binding to SA does not alter reovirus tropism in the brain. Newborn C57/BL6 mice were inoculated intracranially with 100 PFU of either T3D or T3D-σ1R202W (R202W). On day 8 postinoculation, brains of the infected mice were resected. The brains were bisected, the left hemispheres were processed for histopathology, and the right hemispheres were prepared for viral titer determination by plaque assay. Consecutive coronal sections of the brain were stained with hematoxylin and eosin (H&E) or polyclonal reovirus antiserum. Representative sections of brain hemisphere matched for hippocampal depth (A) and cerebellum (B) are shown. Strain T3D-infected brain sections are from the left hemisphere of a brain with a titer of 8 × 10⁸ PFU, and strain T3D-σ1R202W-infected brain sections are from the left hemisphere of a brain with a titer of 3.5 × 10⁸ PFU.