Fig 3.

Size exclusion chromatography of CP(43–233). (A) SDS-PAGE gel showing affinity-purified Ub-CP. (B) Standard curve and size estimation for Ub-CP. A monogram of Ub-CP(43–233) eluted on a Sephacryl G-200 column was constructed by measuring total (solid line) and immunoreactive (dashed line) protein. Solid arrows show the location of the peaks for the BSA, Ub-GFP, and lysozyme standards. The dashed arrow shows the predicted peak for Ub-CP. The asterisks identify minor immunoreactive peaks, which may represent CP multimers. The elution volume of each fraction was 2 ml.