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. 2012 Dec;86(24):13745–13755. doi: 10.1128/JVI.02244-12

TABLE 1.

Primers and probes for quantitative real-time quantitative PCR and RT-PCR

Transcript	Forward primer	Reverse primer	Probe^a
IE2 86	`TGACCGAGGATTGCAACGA`	`CGGCATGATTGACAGCCTG`	`TGGCAGAACTCGGTGACATCCTCGCC`
IE2 exon 5	`GCGCAATATCATGAAAGATAAGAAC`	`GATTGGTGTTGCGAAACATG`	`TCGGCGGGGTGGC`
UL32	`GGTTTCTGGCTCGTGGATGTCG`	`CACACAACACCGTCGTCCGATTAC`	SYBR green
UL55	`AGCTGCGTGACCATCAACCAAACC`	`CTGCGAGTAAAGTTCCAGTACCC`	SYBR green
UL77	`CGTTGCCCGGGAACG`	`GGTGTGAAAGCGGATAAAGGG`	`ACCTAGCTACTTTGGAATCACGCAGAACGA`
UL83	`TCTTCCTGGAGGTACAAGCCA`	`CAGCCACGGGATCGTACTG`	`ACGCGAGACCGTGGAACTGCG`
UL84	`AGACATTGGGACCCTCCGTC`	`GCGGTGATTCGTTCGGG`	`TGGACGATTGGAGCTAG`
α-Tubulin	`AGATGCCAAGTGACAAGACC`	`TCCAACACAAGGTCAATGATCTC`	SYBR green
GAPDH promoter	`TTTCATCCAAGCGTGTAAGGG`	`CAGGACTGGACTGTGGGCA`	`CCCCGTCCTTGACTCCCTAGTGTC`

^a

All TaqMan probes have a 5′ 6-carboxyfluorescein (FAM) and 3′ black hole quencher (BHQ) modification.