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Immunotitration of wheat grain PEPC. Aliquots (75 μL) of an extract (2.7 mg protein/mL) from the dissected aleurone layer of germinating grains were incubated with increasing amounts of sorghum C4-PEPC IgG (•) or preimmune serum (○). PEPC activity remaining in the supernatants was measured as indicated in Methods. One hundred percent activity corresponds to 56 milliunits/mg protein.
