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Immunological localization of PEPC in germinating wheat grains. Grains were soaked for 14 h (A–C) or 24 h (D). Sections (10 μm) were probed with polyclonal PEPC IgGs (1 μg per slide; A, C, and D) or preimmune serum (B). al, Aleurone layer; cr, coleorhiza; ep, epicotyl; ept, epithelium; sc, scutellum; se, starchy endosperm; and r, radicle. Magnifications: A and B, ×50; and C and D, ×100.
