Fig 2.

High-throughput sequencing analysis of RVFV. (A) After incubation with Benzonase nuclease, virion-protected viral RNA was extracted and first-strand cDNA was synthesized. A total of 26 PCR fragments (S-1 to S-4, M-1 to M-8, and L-1 to L-14) were amplified and cloned into a blunt-end plasmid. Ninety-six E. coli colonies transformed with plasmid were picked and transferred into a 96-well plate. A total of 26 96-well plates per virus were analyzed by high-throughput sequencing (Beckman Coulter Genomics). (B) Coverage of sequencing per plate. At least 70 sequence (Seq) reads were obtained for each PCR fragment per 96-well plate.