TABLE 2.

Subpopulation analysis of 23 MP-12 consensus mutations

Segment	Location	MP-12 mutationa			Location at which mutation was observedb	Other ZH548 mutation observed in MP-12 populationc	MP-12 mutation observed
		Nucleotide (antiviral)	Nucleotide (viral)	Amino acid			ZH548 population	Other wt RVFV
S	Intergenic region	G829A	C862U		<MP4j		−	−
	NSs	A1178G	U513C	V160A	MP4		−	−
	NSs	A1288G	U403C		>MP8		−	−
	NSs	A1483G	U208C		MP3		−	−
M	78-kDa protein	U46C	A3840G	I9T			+d	−
	78-kDa protein	G69A	C3817U	V17I			+d	−
	Gn	U795C	A3091G	Y259H		H259L	−	−
	Gn	G857A	C3029U				−	+f
	Gc	A2259U	U1627A	I747L			+e	−
	Gc	A3564G	U322C	R1182G			−	−
	5′ UTR	A3621G	U265C				+	+g
	5′ UTR	A3644G	U242C				−	+h
	5′ UTR	A3660U	U226A				−	−
L	L	C351U	G6054A				−	−
	L	U507C	A5898G		>MP8		−	−
	L	U533C	A5872G	V172A	MP3		−	−
	L	G2130A	C4275U				−	−
	L	G2925A	C3480U				−	−
	L	G3104A	C3301U	R1029K			−	−
	L	A3198G	U3207C				−	−
	L	G3750A	C2655U	M1244I	MP3		−	−
	L	C4368U	G2037A			Y1450C	+	+i
	L	A5208G	U1197C			G1730A	+	−

^aNucleotide mutations in antiviral and viral sense sequences and amino acid mutations in MP-12 sequence compared to parental strain ZH548 sequence.

^bData from Vialat et al. (34).

^cOther amino acid substitution observed in the codon encoding the MP-12 mutation.

^dMutations were located on the same PCR fragment.

^eSixty-three percent of the ZH548 subpopulations encoded this sequence.

^fStrain 1853/78.

^gStrains 2269/74 and 73HB1230.

^hStrains Ken83, ARD-38388, and 2250/74.

ⁱStrain 2373/74.