Fig 6.
Impact of amino acid substitutions that appeared in TPV selection on TPV's dimerization inhibition. COS7 cells were cotransfected with a pair of clones, HIVNL4-3CFP and pHIVNL4-3YFP, carrying E34D (cHIVNL4-3E34D), L63P (cHIVNL4-3L63P), A71V (cHIVNL4-3A71V), K45I (cHIVNL4-3K45I), or V82L (cHIVNL4-3V82L). COS7 cells were further cultured in the continuous presence of 1 or 10 μM TPV, and CFPA/B ratios were determined at the conclusion of the 3-day period of culture. *, not significant; **, P < 0.05.