TABLE 2.
Sensitivities of TPV-resistant variants to various PIsa
| Virus | IC50, mean ± SD (μM) (fold change) |
||||||
|---|---|---|---|---|---|---|---|
| SQV | IDV | APV | LPV | ATV | DRV | TPV | |
| HIV104pre | 0.0043 ± 0.0004 | 0.033 ± 0.002 | 0.031 ± 0.001 | 0.031 ± 0.001 | 0.0036 ± 0.0001 | 0.0035 ± 0.0002 | 0.29 ± 0.03 |
| HIV11MIXP0 | 0.036 ± 0.002 (8) | 0.97 ± 0.007 (29) | 0.31 ± 0.003 (10) | 0.34 ± 0.04 (11) | 0.043 ± 0.005 (12) | 0.015 ± 0.001 (4) | 0.32 ± 0.01 (1) |
| HIV11MIXP5 | 0.21 ± 0.08 (48) | >1 (>30) | 0.32 ± 0.01 (10) | >1 (>32) | 0.38 ± 0.04 (105) | 0.021 ± 0.008 (6) | 2.5 ± 0.01 (8) |
| HIV11MIXP10 | >1 (>233) | >1 (>30) | >1 (>32) | >1 (>32) | >1 (>280) | 0.034 ± 0.004 (10) | >10 (>34) |
a
IC50s were determined by employing MT-4 cells exposed to each virus (50 TCID50s) in the presence of each PI, and the inhibition of p24 Gag protein production was used as the endpoint. All values were determined in triplicate, and the data are shown as mean values ± 1 standard deviation of the results from two or three independent experiments. The numbers in parentheses are changes (n-fold) in the IC50 for each isolate compared to the IC50 of each PI for HIV104pre.