Fig 1.

IFITM3 inhibits infection by VSV-G protein-pseudotyped viruses. (A) Illustration of IFITM3 and its N-terminal mutations. The two transmembrane domains (TM1 and TM2) and the N-terminal sequence of IFITM3 are depicted. Sequences that are mutated in each mutant are also shown. (B) Western blotting to show the expression of IFITM3 and its mutants in stably transduced HEK293 cells. Tubulin was probed as an internal control. (C) Flow cytometry data of one representative infection experiment showing the effect of IFITM3 and its mutants on infection by VSV-G protein-pseudotyped MLV reporter viruses. Control cells were stably transduced with the pQCXIP empty retroviral vector. The percentages of infected cells (GFP positive) are indicated. (D) The relative infectivity was calculated by dividing the percentages of GFP-positive cells from IFITM3-expressing cell lines by the percentage from the control cell line (pQCXIP). The results are the averages of three independent infection experiments. The error bars indicate standard deviations.