Fig 5.

Cellular location of IFITM3 and its mutants. (A) IFITM3 and Δ(1–21) mutant DNAs were transfected into HEK293 cells. Prior to fixation, cells were incubated with Alexa Fluor 555-conjugated transferrin (pseudocolored in red) (5 μg/ml in serum-free Dulbecco modified Eagle medium) for 10 min at 37°C. IFITM3 and the Δ(1–21) mutant were detected by immunostaining with anti-Flag antibodies (pseudocolored in green). (B) IFITM3 and Δ(17–20) and ΔY20 mutant DNAs were transfected into HEK293 cells. Their expression was detected by immunostaining with anti-Flag antibodies (pseudocolored in green). The locations of EEA1, CD63, and TGN46 were visualized by immunostaining with respective antibodies (pseudocolored in red). Representative images are shown.