Fig 5.

Immunocapture PCR assay to detect HSP70-TYLCV CP interaction in viruliferous whiteflies. A TYLCV-specific PCR product. as seen in lanes 1 and 2, was obtained when extracts of dissected midguts of viruliferous whiteflies were applied to PCR tubes coated with an anti-HSP70 specific antibody, and a PCR was performed on the caught complexes (lanes 3 and 4). Control treatments with midgut extracts of nonviruliferous whiteflies (lanes 5 and 6) and with uncoated tubes (lanes 7 and 8) were negative.