TABLE 1.

Differentially transcribed ESTs in the midgut of B. tabaci following TYLCV and SLCV acquisition and retention^a

EST clone	Best hit in GenBank	GenBank accession no.	GO term	Biological function	Microarray expression in midgut
					TYLCV	SLCV
HBT008_F09_T3_078	70-kDa heat shock	EE597405	000940	Response to heat, protein folding	1.65	1.51
TOMOV-BT003_A05	OS-D-like	EE599211	0009700	Sensory perception	2.81
BT-H-034-1-H5-T3_H05	Pantothenate kinase 4	EE596485	0015937	Coenzyme A biosynthesis		5.74
BT-H-032-1-C9-T3_C09	Transmembrane assembly	EE596401	0006928	Cellular component movement		3.96
BT-TYLCV-053-1-H9-T3_H09	Checkpoint-like	EE601807	0000075	Cell cycle checkpoint		3.22
BT-H-020-1-E2-T3_E02	Cathepsin B protease	EE595866	000691	Autophagy, proteolysis response to stress		2.09
BT-TYLCV-018-1-H4-T3_H04	Sugar transporter	EE600420	000535	Glucose transmembrane transporter		2.01
BT-TOMOV-040-1-G8-T3_G08	Btk-1	EE598908		Immunity		1.59
HBT006_G06_T3_051	Cathepsin F protease	EE597298	000658	Proteolysis, receptor-mediated endocytosis		1.59
BT-TYLCV-056-1-F6-T3_F06	ATP synthase	EE601889	0042776	ATP synthesis-coupled proton transport	-1.7	-2.6
BT-TOMOV-042-1-F9-T3_F09	NADH dehydrogenase subunit 1	EE599001	0005747	Mitochondrial respiratory chain complex I		2.7
BT-TYLCV-057-1-E4-T3_E04	Nuclear envelope membrane protein	EE601922	001503	Transmembrane transport		2.58
BT-TYLCV-051-1-A8-T3_A08	Fatty acid binding protein	EE601683	000521	Transporter activity		1.97
TOMOV-BT012_G05	Sugar transporter	EE599789	000535	Glucose transmembrane transporter activity		2.01
BT-HINST-004-1-C2-T3_C02	ATP synthase subunit C	EE602876	0040007	Cytoplasmic membrane-bounded vesicle		1.88
TOMOV-BT012_C02	Phosphoglycerate mutase 1	EE599756	0006096	Glycolysis		1.55
BT-TYLCV-045-1-A6-T3_A06	Cytochrome c oxidase subunit III	EE601430	0006123	Mitochondrial electron transport		1.55
BT-TOMOV-028-1-A9-T3_A09	Adenine nucleotide translocator	EE598307	0055085	Transmembrane transport		1.54
BT-TYLCV-042-1-B2-T3_B02	ATP-binding FecE	EE601334	0006811	Ion transport		1.53
BT-TYLCV-018-1-H4-T3_H04	Acyl-coenzyme A esterase	EE600420	0004091	Protein binding		2.01
BT-TYLCV-051-1-A8-T3_A08	Putative fatty acid binding protein	EE601683	000521	Transporter activity		1.97
TOMOV-BT012_C02	Phosphoglycerate mutase 1	EE599756	0006096	Glycolysis		1.55
BT-TOMOV-020-1-D2-T3_D02	Vitellogenin	EE597946	0006869	Lipid transport		1.67
TYLCV006_F04	Cytochrome P450 CYP6CX2	EE602200	0055114	Oxidation reduction	1.78
BT-TYLCV-058-1-F5-T3_F05	Cytochrome oxidase I	EE601978	000612	Mitochondrial electron transport	−1.78
BT-TOMOV-028-1-E7-T3_E07	Sodium-dependent glucose transporter	EE598330	000864	Carbohydrate transport	−1.63
BT-HINST-020-1-B11-T3_B11	Apolipophorin III	EE603648	0008289	Lipid binding	−1.69
BT-TYLCV-025-1-B7-T3_B07	Cytochrome c oxidase subunit II	EE600708	000609	Precursor metabolites and energy	−1.57

^aEST (expressed sequence tag) clone name, best hit in GenBank, GenBank accession no. of the EST, GO (gene ontology) category, biological function of the expected protein, and the average ratio of three highly correlated biological replicates following the microarray experiments comparing viruliferous and nonviruliferous midguts with TYLCV or SLCV are shown.