Fig 5.

EBV-PK is not required for early lytic protein expression induced by hydrogen peroxide. (A) AGS-WT and PKmut cells were generated and transfected with the indicated combinations of Z, R, and EBV-PK expression vectors, and Western blot analysis was performed to detect hyper- and hypophosphorylated BMRF1. Antibodies against Z and β-actin were also included as controls. (B) AGS-PKmut cells were treated with or without H₂O₂ (300 μM) in the presence or absence of the ATM inhibitor KU55933 (10 μM), and Western blot analysis using antibody against BMRF1 or Z was used to detect early lytic protein expression. β-Actin was included as a loading control.