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Gag sequences of M250I mutants from the acute subtype C cohort. An alignment of p24 sequences of viruses carrying M250I mutation at transmission shows the reversion over time in three participants (red asterisk [CAP255, CAP261, and CAP277]). The B*57/B*58:01-restricted TW10 epitope is highlighted in gray. The CypA binding loop is underlined. Site 250 is highlighted in yellow, whereas sites 252 and 260 are highlighted in green.
