Fig 6.

M250I compensation in subtype C and B viruses. (A) Replication capacities of site-directed mutants (T242N, M250I, M250I/S252G/D260E [IGE] and T242N/M250I/S252G/D260E [NIGE]) in a subtype C backbone showing the compensatory effect of the S252G and D260E mutations on the M250I mutant (IGE). Pooled CD8-depleted PBMC were inoculated in duplicate at an MOI of 0.002, and viral spread was measured by qPCR. The error bars represent standard errors for duplicate measurements. (B) Stratification of the subtype C Gag-protease replication capacities according to the number of the compensatory mutations (S252G and D260E). (C) Replication capacities of subtype B NL4-3 site mutants showing the minimal compensatory effect of the G248A mutation. The error bars represent the standard errors for triplicate measurements.