Fig 2.

BEFV infection impaired by inhibition of dynamin 2 activity. (A) MDBK and Vero cells were pretreated with different amounts of Dynasore for 1 h and infected with BEFV at an MOI of 2. The cell lysates and supernatants of BEFV-infected cells were collected at 24 hpi for Western blotting and viral titration, respectively. Cell lysates were analyzed by Western blotting using anti-BEFV M antibody. The results are from three triplicate experiments; error bars indicate the means ± standard deviations. (B) BEFV infection is inhibited in cells transfected with shRNAs specific to dynamin 2. MDBK and Vero cells were transfected with dynamin 2 shRNA and mock control vectors (scrambled pGFP-V-RS and pGFP-V-RS), respectively, for 24 h and then infected with BEFV at an MOI of 2. The cell lysates and supernatants of BEFV-infected cells were collected at 24 hpi for Western blotting and viral titration, respectively. The cell lysates were analyzed by Western blotting using anti-dynamin 2, anti-BEFV M, and anti-β-actin antibodies. The β-actin was included as an internal control for normalization.