Fig 3.

The effect of clathrin and dynamin 2 inhibitors or shRNAs on viral transcription. MDBK cells were pretreated with different inhibitors, respectively, for 1 h or transfected with specific clathrin or dynamin 2 shRNAs for 24 h and then infected with BEFV at an MOI of 2. The BEFV-infected cells were collected at 24 hpi, and total RNAs were extracted for semiquantitative RT-PCR. The inhibitors and shRNAs used are indicated on the top of the gel. The GAPDH gene was used as an internal control for normalization. The levels of M and GAPDH mRNA were compared. Numbers below each lane are percentages of the control level of a specific protein in mock-treated cells. Similar results were obtained from three independent experiments.