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. Author manuscript; available in PMC: 2012 Nov 21.
Published in final edited form as: Int J Toxicol. 2011 Oct 12;30(6):619–638. doi: 10.1177/1091581811422413

Figure 11.

Figure 11

N,N′-bis(2-Mercaptoethyl)isopthalamide (NBMI) protects against the mercury- and oxidant-induced glutathione (GSH) loss. Mouse aortic endothelial cells ([MAECs] 5 × 105 cells/35 mm dish) pretreated with minimal essential medium (MEM) alone or MEM containing NBMI (50 μmol/L) for 1 hour were treated with MEM alone or MEM containing methylmercury (10 μmol/L) or thimerosal (25 μmol/L) or H2O2 (100 μmol/L) or diamide (250 μmol/L). At the end of the incubation period, the intracellular soluble thiol (glutathione [GSH]) concentrations were determined. Data represent mean ± standard deviation (SD) calculated from 3 independent experiments. *Significantly different at P < .05 as compared to cells treated with MEM alone. **Significantly different at P < .05 as compared to cells treated with MEM containing mercury or oxidant alone.