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. Author manuscript; available in PMC: 2012 Nov 21.
Published in final edited form as: Int J Toxicol. 2011 Oct 12;30(6):619–638. doi: 10.1177/1091581811422413

Figure 7.

Figure 7

N,N′-bis(2-Mercaptoethyl)isopthalamide (NBMI) attenuates the mercury-induced phospholipase D1 (PLD1) and PLD2 in situ translocation. Mouse aortic endothelial cells (MAECs) cultured on coverslips (5 × 105 cells/35 mm dish) were pretreated with minimal essential medium (MEM) alone or MEM containing NBMI (50 μmol/L) for 1 hour then treated with MEM alone or MEM containing methylmercury (5 and 10 μmol/L) and thimerosal (10 and 25 μmol/L) for 1 hour. At the end of the incubation period, the cells were fixed, stained for PLD1 (A and B) and PLD2 (C and D), and visualized using confocal fluorescent microscopy. Each digitally captured image is a representative picture obtained from 3 independent experiments conducted under identical conditions.