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. Author manuscript; available in PMC: 2012 Nov 21.
Published in final edited form as: Int J Toxicol. 2011 Oct 12;30(6):619–638. doi: 10.1177/1091581811422413

Figure 8.

Figure 8

Mercury induces phosphorylation of extracellular-regulated kinase 1/2 (ERK1/2) and phospholipase D1 (PLD1) in mouse aortic endothelial cells (MAECs). The MAECs (5 × 105 cells/35 mm dish) were treated with minimal essential medium (MEM) alone or MEM containing methylmercury (10 μmol/L) for 5, 15, and 30 minutes (A and B) and MEM containing thimerosal (25 μmol/L) for 5 and 60 minutes (C and D). Following treatment, proteins in cell lysates were subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting with phospho-ERK1/2-specific, ERK1/2-specific, phosphotheonine-PLD1-specific, and PLD1-specific monoclonal antibodies. The intensity of the protein bands were digitally determined as described in Materials and Methods. *Significantly different at P < .05 as compared to cells treated with MEM alone.