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. Author manuscript; available in PMC: 2012 Nov 21.
Published in final edited form as: Int J Toxicol. 2010 Dec 3;30(1):69–90. doi: 10.1177/1091581810388850

Figure 11.

Figure 11

Bleomycin induces phosphatidic acid (PA) formation in ECs. Bovine lung microvascular ECs ([BLMVECs] 5 × 105 cells/35-mm dish) were labeled with [32P]orthophosphate in DMEM phosphate-free medium for 12 hours. Following [32P]orthophosphate labeling, cells were treated with MEM alone or MEM containing different concentrations (5, 10, and 100 μg) of bleomycin for 12 hours. At the end of the incubation period, [32P]PA formed was determined from the autoradiogram (A) and quantified from intensity (B). Data represent mean ± SD calculated from 3 independent experiments. *Significantly different at P < .05 as compared to cells treated with MEM alone. DMEM, Dulbecco modified Eagle medium; MEM, minimal essential medium; ECs, endothelial cells.