Skip to main content
. Author manuscript; available in PMC: 2012 Nov 21.
Published in final edited form as: Int J Toxicol. 2010 Dec 3;30(1):69–90. doi: 10.1177/1091581810388850

Figure 7.

Figure 7

Bleomycin induces lipid peroxidation in ECs. Bovine lung microvascular ECs [BLMVECs] on coverslips (5 × 105 cells/35-mm dish) were subjected to treatment with MEM alone or MEM containing bleomycin (5, 10, and 100 μg) for 12 hours to assay the extent of lipid peroxidation. At the end of the incubation period, the cells were fixed, stained for 4-HNE formation, and visualized using confocal fluorescence microscopy. The fluorescence intensities were measured. Each micrograph is a representative picture obtained from 3 independent experiments conducted under identical conditions. Data represent mean ± SD calculated from 3 independent experiments. *Significantly different at P < .05 as compared to cells treated with vehicle alone. 4-HNE indicates 4-hydroxy-2-nonenal; MEM, minimal essential medium; ECs, endothelial cells.