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. Author manuscript; available in PMC: 2012 Nov 21.
Published in final edited form as: Int J Toxicol. 2010 Dec 3;30(1):69–90. doi: 10.1177/1091581810388850

Figure 9.

Figure 9

Bleomycin induces cytotoxicity in ECs. Bovine lung microvascular ECs ([BLMVECs] 2.5 × 105 cells/17.5-mm dish) were subjected to treatment with MEM alone or MEM containing bleomycin (5, 10, and 100 μg) for 8 (A), 12 (B), and 24 (C) hours. At the end of the incubation period, the release of LDH into the medium (as an index of cytotoxicity) was determined spectrophotometrically. Data represent mean ± SD calculated from 3 independent experiments. *Significantly different at P < .05 as compared to cells treated with MEM alone. LDH, indicates lactate dehydrogenase; MEM, minimal essential medium; ECs, endothelial cells.