Skip to main content
. Author manuscript; available in PMC: 2012 Nov 21.
Published in final edited form as: Nat Rev Microbiol. 2008 Nov;6(11):851–857. doi: 10.1038/nrmicro2014

Figure 1. Mycobacteriophage-encoded recombination proteins.

Figure 1

A. Mycobacteriophages Che9c gp60 and gp61 are E. coli Rac prophage RecE and RecT homologues, respectively. Mycobacteriophage Halo encodes a RecE homologue that is 46% identical to Che9c gp60, while Giles gp52 belongs to the YqaJ family of exonucleases. Halo gp43 is similar to Giles gp53, but these are only distantly related to other phage RecT-like proteins. The λ Red recombination proteins are not closely related to the mycobacteriophage proteins or RecET.

B. Electron micrograph of Che9c gp61 protein multimers in the presence of ssDNA. Reactions containing gp61 protein (1.2 µM) incubated with ssDNA (100 nt; 2 µM) were absorbed to glow discharged 400 mesh formvar carbon coated copper grids, stained with 2% uranyl acetate, and examined by transmission electron microscopy. Images were collected at a magnification of 140,000×; four examples of toroid structures are shown alongside a size bar.