Fig. 1.
Effect of eIF4G1, eIF4G2 and eIF4E silencing on overall protein synthesis and cell survival in IR-treated cells. (A) NS (non-silencing) and eIF4G1-silenced MCF10A cells were treated with 8 Gy IR or mock-treated. Protein synthesis rates determined by [35S]methionine incorporation, normalized to NS-shRNA nonirradiated control (0 h). SEMs are shown. Immunoblot is eIF4G1 in silenced and control cells. (B) Equal amounts of protein at 24 h post-IR were resolved by SDS/PAGE and immunoblotted with the indicated antibodies. (C) Clonogenic survival assays performed on MCF10A cells following IR, with stable shRNA to eIF4E, eIF4G1, eIF4G2, or an NS random sequence. Cells were plated overnight and irradiated, and colonies were scored after 2 wk (n = 3). (D) Immunoblot of equal protein amounts of lysates from C.