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. 2012 Oct 29;109(46):19003–19008. doi: 10.1073/pnas.1209590109

Fig. 4.

Fig. 4.

BRET analyses and cAMP assays illustrating that GHS-R1a forms constitutive heteromers with SST5 but not with SST2 and blocks SST/SST5-induced suppression of cAMP accumulation. The BRET ratio is expressed as a function of the acceptor/donor DNA ratio and is defined as [(emission at 515 nm) − (background emission at 515 nm)]/[(emission at 410 nm) − (background emission at 410 nm)]. (A) The BRET ratio in HEK293 cells cotransfected with 0.1 μg GHS-R1a-Rluc and increasing amounts (0.1–1.8 μg) of SST5-GFP (upper curve; BRET50 = 2) or 0.1 μg SST5-Rluc DNA and increasing amounts (0.1–1.8 μg) of SST5-GFP DNA (lower curve). (B) The BRET ratio measured in HEK293 cells cotransfected with 0.1 μg GHS-R1a-Rluc and 0.1–1.8 μg ST2-GFP. (C) HEK293 cells coexpressing GHS-R1a and SST5 are refractory to SST suppression of forskolin-induced cAMP accumulation. (D) SST inhibition of forskolin-induced cAMP accumulation in HEK293 cells coexpressing GHS-R1a and SST2.