Fig. 1.

RAB-5 and RAB-10 are regulators of DCV secretion. (A) Schematic describing the NLP-21-YFP assay used for analyzing DCV secretion in C. elegans. (B) All rab GTPase mutants were systematically tested for defects in NLP-21-YFP secretion. For Rabs where no mutant was available, tissue-specific knockdown was conducted in DA and DB cholinergic motoneurons (indicated by *) or a dominant-active variant was expressed and analyzed. Note: RNAi experiments were normalized to knockdown of mock vector (L4440). Error bars indicate SEM. ***P < 0.001; **P < 0.01; *P < 0.05 (one-way ANOVA with Bonferroni post test). Representative pictures are shown in Fig. S1. (C) Secretion of NLP-21-YFP is impaired in rab-5 and rab-10 mutants Error bars indicate SEM. ***P < 0.001; (one-way ANOVA with Bonferroni post test). (D and E) NLP-21-YFP fluorescence levels in the DNC are unaffected (D), whereas fluorescence levels in the coelomocytes are decreased (E). Tissue-specific knockdown of rab-5 and rab-10 in neurons showed similar defects in secretion. [Scale bars: 6 μm (in DNC); 4 μm (in coelomocytes).]